Hotspot

trnN-GUU-trnR-ACG

IRa · 133248-133924 · 677 bp

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Pi 0.00197

MarkerSeek score 60.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 677
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	782 bp	1.0	77.3
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	769 bp	1.0	77.2
trnN-GUU-trnR-ACG_p3	TAGAATCTCCCGCTGTTCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	827 bp	1.0	77.2
trnN-GUU-trnR-ACG_p4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	770 bp	1.0	76.8
trnN-GUU-trnR-ACG_p5	GCTGTTAACTGACTGGTCGT	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	819 bp	1.0	76.7