Hotspot

ndhF-rpl32

SSC · 115493-116228 · 736 bp

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Pi 0.00272

MarkerSeek score 55.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 3
pi: 0.00272
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAGTAGAAGTCCAGCCCCT	GCCCAATATCCCCGCTTTTT	50.0	50.0	58.0	58.9	901-1103 bp	1.0	73.1
ndhF-rpl32_p2	AAAGTAGAAGTCCAGCCCCT	CTTTCAGCGCTGCCCAATAT	50.0	50.0	58.0	59.0	912-1114 bp	1.0	72.3
ndhF-rpl32_p3	AAAGTAGAAGTCCAGCCCCT	TTTCAGCGCTGCCCAATATC	50.0	50.0	58.0	59.0	911-1113 bp	1.0	72.3
ndhF-rpl32_p4	AAAGTAGAAGTCCAGCCCCT	CCAATATCCCCGCTTTTTCC	50.0	50.0	58.0	56.8	899-1101 bp	1.0	70.7
ndhF-rpl32_p5	AAAGTAGAAGTCCAGCCCCT	CCCAATATCCCCGCTTTTTC	50.0	50.0	58.0	56.8	900-1102 bp	1.0	70.7