Hotspot

rpl16-rps3

LSC · 84137-85350 · 1214 bp

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Pi 0.00165

MarkerSeek score 59.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 3
pi: 0.00165
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GCCCTGCTTCGATTTGTCTA	AGGGTTCCTCTCCAAACCAT	50.0	50.0	58.0	58.2	1427-1429 bp	1.0	78.1
rpl16-rps3_p2	GCCCTGCTTCGATTTGTCTA	GAAGGTAGGGTTCCTCTCCA	50.0	55.0	58.0	57.8	1433-1435 bp	1.0	78.0
rpl16-rps3_p3	GCCCTGCTTCGATTTGTCTA	TAGGGTTCCTCTCCAAACCA	50.0	50.0	58.0	57.6	1428-1430 bp	1.0	76.6
rpl16-rps3_p4	GCCCTGCTTCGATTTGTCTA	AGAAGGTAGGGTTCCTCTCC	50.0	55.0	58.0	57.4	1434-1436 bp	1.0	75.6
rpl16-rps3_p5	GCCCTGCTTCGATTTGTCTA	GGGTTCCTCTCCAAACCATT	50.0	50.0	58.0	57.4	1426-1428 bp	1.0	75.2