Hotspot

rpl36-rps8

LSC · 82218-82706 · 489 bp

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Pi 0.00477

MarkerSeek score 59.60

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 3
pi: 0.00477
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TCCCCTTCTACGGATCAGTC	CTCTCGACTTCTCGGGGTAT	55.0	55.0	57.9	58.0	635-636 bp	1.0	79.7
rpl36-rps8_p2	TCCCCTTCTACGGATCAGTC	GAAGGAATCGGCGGAGAAAT	55.0	50.0	57.9	58.0	590-591 bp	1.0	79.6
rpl36-rps8_p3	TCTTTGTTTATGTCGCGGGT	CTCTCGACTTCTCGGGGTAT	45.0	55.0	57.8	58.0	677-678 bp	1.0	78.9
rpl36-rps8_p4	ACGGATCAGTCGGCATTTTT	CTCTCGACTTCTCGGGGTAT	45.0	55.0	58.2	58.0	626-627 bp	1.0	78.8
rpl36-rps8_p5	TCTTTGTTTATGTCGCGGGT	GAAGGAATCGGCGGAGAAAT	45.0	50.0	57.8	58.0	632-633 bp	1.0	78.8