Hotspot

trnF-GAA-ndhJ

LSC · 50056-50890 · 835 bp

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Pi 0.0032

MarkerSeek score 60.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 4
pi: 0.0032
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	941-945 bp	1.0	79.3
trnF-GAA-ndhJ_p2	TGTCGGTAATGGTCGGGATA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	991-995 bp	1.0	79.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGGCTGAA	50.0	50.0	58.1	58.2	980-984 bp	1.0	78.4
trnF-GAA-ndhJ_p4	TGTCGGTAATGGTCGGGATA	GATAATCATCCGCGGCTGAA	50.0	50.0	57.9	58.2	1030-1034 bp	1.0	78.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATAATCATCCGCGGCTGAAA	50.0	45.0	58.1	57.7	979-983 bp	1.0	78.0