Hotspot

trnG-UCC-trnfM-CAU

LSC · 38259-38444 · 186 bp

Back to Sapindus

Pi 0.00358

MarkerSeek score 60.50

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 1
pi: 0.00358
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	255-264 bp	1.0	81.7
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	291-300 bp	1.0	81.4
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	253-262 bp	1.0	80.6
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	276-285 bp	1.0	80.0
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	275-284 bp	1.0	80.0