Hotspot

trnK-UUU-rps16

LSC · 4437-5270 · 834 bp

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Pi 0

MarkerSeek score 59.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCGACAGTATGGACGAATC	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	57.9	58.0	990-999 bp	1.0	79.0
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	928-937 bp	1.0	78.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	927-936 bp	1.0	78.7
trnK-UUU-rps16_p4	TCCCGACAGTATGGACGAAT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	991-1000 bp	1.0	78.3
trnK-UUU-rps16_p5	ACTCTCCCGACAGTATGGAC	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	58.2	58.0	995-1004 bp	1.0	78.2