Hotspot

ycf1

SSC · 112907-118603 · 5697 bp

Back to Santalum

Pi 0.00689

MarkerSeek score 45.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5697
variable_sites: 147
pi: 0.00689
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCCCTTTTCTTGTTCTGG	TCTCACCGATTCCCGAGAAT	45.0	50.0	54.9	58.2	171-180 bp	1.0	57.4
ycf1_p2	TGCTCTATCTTCAGAATCTCCCT	TCTCACCGATTCCCGAGAAT	43.5	50.0	58.2	58.2	187-196 bp	1.0	56.6
ycf1_p3	TGCTCTATCTTCAGAATCTCCC	TCTCACCGATTCCCGAGAAT	45.5	50.0	56.8	58.2	187-196 bp	1.0	56.3
ycf1_p4	TCTCCCTTTTCTTGTTCTGG	TTCTCACCGATTCCCGAGAA	45.0	50.0	54.9	58.4	172-181 bp	1.0	55.6
ycf1_p5	TCTCCCTTTTCTTGTTCTGG	TTCTTCTCACCGATTCCCGA	45.0	50.0	54.9	58.4	175-184 bp	1.0	55.5