Hotspot

rpl22

LSC · 83103-83582 · 480 bp

Back to Santalum

Pi 0.00625

MarkerSeek score 35.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 480
variable_sites: 12
pi: 0.00625
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GGGAATGATGCTCATGGGTT	AATTAACAGGAGGCGAACTT	50.0	40.0	57.9	55.0	548 bp	0.9	53.1
rpl22_p2	GGGAATGATGCTCATGGGTT	ATTAACAGGAGGCGAACTTT	50.0	40.0	57.9	55.0	547 bp	0.9	53.1
rpl22_p3	TGCTCATGGGTTGTACCAAG	AATTAACAGGAGGCGAACTT	50.0	40.0	57.8	55.0	540 bp	0.9	52.1
rpl22_p4	TGCTCATGGGTTGTACCAAG	ATTAACAGGAGGCGAACTTT	50.0	40.0	57.8	55.0	539 bp	0.9	52.1
rpl22_p5	GCTCATGGGTTGTACCAAGT	ATTAACAGGAGGCGAACTTT	50.0	40.0	57.8	55.0	538 bp	0.9	52.1