Hotspot

infA

LSC · 79516-79754 · 239 bp

Back to Santalum

Pi 0.015

MarkerSeek score 41.80

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 239
variable_sites: 10
pi: 0.015
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TGAACCTCGAATTCTATCCCC	ACTCCTACTCCACGAGTTGA	47.6	50.0	56.9	57.4	343-353 bp	1.0	63.7
infA_p2	TGAACCTCGAATTCTATCCCC	TCACTCCTACTCCACGAGTT	47.6	50.0	56.9	57.4	345-355 bp	1.0	63.7
infA_p3	GAACCTCGAATTCTATCCCCA	ACTCCTACTCCACGAGTTGA	47.6	50.0	56.9	57.4	342-352 bp	1.0	63.5
infA_p4	GAACCTCGAATTCTATCCCCA	TCACTCCTACTCCACGAGTT	47.6	50.0	56.9	57.4	344-354 bp	1.0	63.4
infA_p5	GAACCTCGAATTCTATCCCCA	CACTCCTACTCCACGAGTTG	47.6	55.0	56.9	57.4	343-353 bp	1.0	63.0