Hotspot

ndhD-psaC

SSC · 110738-110836 · 99 bp

Back to Santalum

Pi 0.0364

MarkerSeek score 58.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 99
variable_sites: 18
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAGAAACCCACAAATATCAGA	GCCTGTCCAACCGATTTCTT	34.8	50.0	55.6	58.5	256-263 bp	1.0	45.9
ndhD-psaC_p2	AGAAGAAACCCACAAATATCAGA	AAGAGATGTGAATCCGCCTG	34.8	50.0	55.6	57.7	271-278 bp	1.0	45.6
ndhD-psaC_p3	AGAAGAAACCCACAAATATCAGA	GAGATGTGAATCCGCCTGTC	34.8	55.0	55.6	58.4	269-276 bp	1.0	45.6
ndhD-psaC_p4	AGAAGAAACCCACAAATATCAGAA	AAGAGATGTGAATCCGCCTG	33.3	50.0	56.3	57.7	271-278 bp	1.0	45.6
ndhD-psaC_p5	AAGAAGAAACCCACAAATATCAGA	AAGAGATGTGAATCCGCCTG	33.3	50.0	56.3	57.7	272-279 bp	1.0	45.5