Hotspot

petA-psbJ

LSC · 62817-63635 · 819 bp

Back to Santalum

Pi 0.0125

MarkerSeek score 64.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 30
pi: 0.0125
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCAGGGCCTTTTGTTCTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.0	58.0	771-1074 bp	1.0	86.2
petA-psbJ_p2	TCCAGGGCCTTTTGTTCTTC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.0	58.0	772-1075 bp	1.0	86.2
petA-psbJ_p3	GGCGTCTGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.0	58.0	750-1053 bp	1.0	86.1
petA-psbJ_p4	CCAGGGCCTTTTGTTCTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.0	58.1	772-1075 bp	1.0	85.6
petA-psbJ_p5	TCCAGGGCCTTTTGTTCTTC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.0	58.1	773-1076 bp	1.0	85.5