Hotspot

ycf4-cemA

LSC · 60233-60942 · 710 bp

Back to Santalum

Pi 0.00419

MarkerSeek score 61.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 10
pi: 0.00419
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AAGAGAGAGATCCACCAGGG	45.0	55.0	58.1	57.8	461-833 bp	1.0	82.9
ycf4-cemA_p2	GAACAAAAAGCCGCCGAATT	AAGAGAGAGATCCACCAGGG	45.0	55.0	58.2	57.8	479-851 bp	1.0	81.8
ycf4-cemA_p3	ACTCCACGCGAAATTGAACA	AAGAGAGAGATCCACCAGGG	45.0	55.0	58.4	57.8	494-866 bp	1.0	80.3
ycf4-cemA_p4	AACAAAAAGCCGCCGAATTG	AAGAGAGAGATCCACCAGGG	45.0	55.0	58.5	57.8	478-850 bp	1.0	79.6
ycf4-cemA_p5	AATTGAACAAAAAGCCGCCG	AAGAGAGAGATCCACCAGGG	45.0	55.0	58.5	57.8	483-855 bp	1.0	79.6