Hotspot

rbcL-accD

LSC · 56314-56956 · 643 bp

Back to Santalum

Pi 0.00585

MarkerSeek score 61.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 17
pi: 0.00585
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CCCACATCCGTGTTCAAACT	55.0	50.0	58.0	58.4	827-845 bp	1.0	80.3
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TACTTAGCCCACATCCGTGT	55.0	50.0	58.0	58.4	834-852 bp	1.0	80.0
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CCCACATCCGTGTTCAAACT	50.0	50.0	57.9	58.4	797-815 bp	1.0	79.9
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TACTTAGCCCACATCCGTGT	50.0	50.0	57.9	58.4	804-822 bp	1.0	79.5
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CTTAGCCCACATCCGTGTTC	55.0	55.0	58.0	58.6	832-850 bp	1.0	78.4