Hotspot

ycf3-trnS-GGA

LSC · 45722-46477 · 756 bp

Back to Santalum

Pi 0.00961
MarkerSeek score 67.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
756
variable_sites
30
pi
0.00961
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCTTGAAGTTGTCGGAGT TGCTACGCCTTTAACCACTC 45.0 50.0 56.1 57.9 819-969 bp 1.0 71.1
ycf3-trnS-GGA_p2 TGCTTCCCTTTCTCTTGAAGT TGCTACGCCTTTAACCACTC 42.9 50.0 57.1 57.9 829-979 bp 1.0 71.0
ycf3-trnS-GGA_p3 TCTCTTGAAGTTGTCGGAGT CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.1 58.1 854-1004 bp 1.0 70.9
ycf3-trnS-GGA_p4 TGCTTCCCTTTCTCTTGAAGT CCCTCGGTAAACAAAAGCCT 42.9 50.0 57.1 58.1 864-1014 bp 1.0 70.9
ycf3-trnS-GGA_p5 TCTCTTGAAGTTGTCGGAGT ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.1 58.2 875-1025 bp 1.0 70.4