Hotspot

trnT-GGU-psbD

LSC · 32992-34347 · 1356 bp

Back to Santalum

Pi 0.00538

MarkerSeek score 59.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1356
variable_sites: 33
pi: 0.00538
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ATCCGCACAGTGACTCATTC	CTGCGTAACCAGTCATCCAT	50.0	50.0	58.0	57.7	1539-1556 bp	1.0	79.6
trnT-GGU-psbD_p2	ATCCGCACAGTGACTCATTC	ACCTACAAAAACGAACCGGT	50.0	45.0	58.0	57.7	1561-1578 bp	1.0	79.4
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CTGCGTAACCAGTCATCCAT	55.0	50.0	58.1	57.7	1485-1502 bp	1.0	79.2
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAACCGGT	55.0	45.0	58.1	57.7	1507-1524 bp	1.0	79.0
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CTGCGTAACCAGTCATCCAT	50.0	50.0	58.1	57.7	1488-1505 bp	1.0	79.0