Hotspot

petN-psbM

LSC · 29300-30383 · 1084 bp

Back to Santalum

Pi 0.0092

MarkerSeek score 65.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 41
pi: 0.0092
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATCGCTTGGGCTGCTTTAAT	ATTGCTACTGCGCTGTTCAT	45.0	45.0	58.2	58.3	1248-1295 bp	1.0	79.5
petN-psbM_p2	ATGGGGGAGAAATGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.7	58.3	1195-1242 bp	1.0	79.1
petN-psbM_p3	GTATCGCTTGGGCTGCTTTA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.3	58.3	1250-1297 bp	1.0	78.6
petN-psbM_p4	CACTCGTAGTATGGGGGAGA	ATTGCTACTGCGCTGTTCAT	55.0	45.0	57.6	58.3	1205-1252 bp	1.0	78.6
petN-psbM_p5	ATCGCTTGGGCTGCTTTAAT	TATTGCTACTGCGCTGTTCA	45.0	45.0	58.2	57.6	1249-1296 bp	1.0	78.5