Hotspot

rps2-rpoC2

LSC · 17054-17210 · 157 bp

Back to Santalum

Pi 0.0183

MarkerSeek score 62.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 6
pi: 0.0183
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AAAATGCACTCCTGCTTCCA	TTTTGTTCTACCACAGAGGA	45.0	40.0	58.0	53.8	318-323 bp	1.0	48.2
rps2-rpoC2_p2	AAATGCACTCCTGCTTCCAT	TTTTGTTCTACCACAGAGGA	45.0	40.0	57.8	53.8	317-322 bp	1.0	46.4
rps2-rpoC2_p3	AATGCACTCCTGCTTCCATC	TTTTGTTCTACCACAGAGGA	50.0	40.0	58.2	53.8	316-321 bp	1.0	46.3
rps2-rpoC2_p4	AAAATGCACTCCTGCTTCCA	TTGTTCTACCACAGAGGATT	45.0	40.0	58.0	53.6	316-321 bp	1.0	46.3
rps2-rpoC2_p5	AAAATGCACTCCTGCTTCCA	TTTGTTCTACCACAGAGGAT	45.0	40.0	58.0	53.6	317-322 bp	1.0	46.3