Hotspot

atpF-atpH

LSC · 13907-14071 · 165 bp

Back to Santalum

Pi 0.00976

MarkerSeek score 57.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 5
pi: 0.00976
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TATTAAACCCGAAACCCCCG	GCAGAGGGAAAGATACGAGG	50.0	55.0	57.6	57.5	343-358 bp	1.0	77.1
atpF-atpH_p2	GGCAGATGACCAGTGACCTA	GCAGAGGGAAAGATACGAGG	55.0	55.0	58.8	57.5	324-339 bp	1.0	74.3
atpF-atpH_p3	GCAGATGACCAGTGACCTAA	GCAGAGGGAAAGATACGAGG	50.0	55.0	56.9	57.5	323-338 bp	1.0	72.2
atpF-atpH_p4	TATTAAACCCGAAACCCCCG	CCGAAGCAGAGGGAAAGATAC	50.0	52.4	57.6	58.2	348-363 bp	1.0	72.0
atpF-atpH_p5	ACACCGAGCACTACACTTAG	GCAGAGGGAAAGATACGAGG	50.0	55.0	57.0	57.5	392-407 bp	1.0	71.8