Hotspot

trnK-UUU-rps16

LSC · 4348-5140 · 793 bp

Back to Santalum

Pi 0.00704

MarkerSeek score 57.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 18
pi: 0.00704
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCCTTGCTTCATCCA	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	871-910 bp	1.0	84.8
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	872-911 bp	1.0	84.8
trnK-UUU-rps16_p3	ATGGACGAATCCCTTGCTTC	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	876-915 bp	1.0	84.8
trnK-UUU-rps16_p4	GGACGAATCCCTTGCTTCAT	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.0	58.1	874-913 bp	1.0	84.8
trnK-UUU-rps16_p5	CGATGGTATGGACGAATCCC	GGGCGGAGGTTTTTAAGGAA	55.0	50.0	57.9	58.1	883-922 bp	1.0	84.5