Hotspot

rpl14

LSC · 80344-80712 · 369 bp

Back to Sansevieria

Pi 0.00136

MarkerSeek score 27.10

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TCAGCAATAACGTCTCTGCC	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	1019 bp	1.0	80.5
rpl14_p2	TCAGCAATAACGTCTCTGCC	GACAAATAGAAGCAGGGCGA	50.0	50.0	58.0	58.0	978 bp	1.0	80.5
rpl14_p3	CGGGTCGACTGATACGTTTT	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	1246 bp	1.0	80.3
rpl14_p4	CGGGTCGACTGATACGTTTT	GACAAATAGAAGCAGGGCGA	50.0	50.0	58.0	58.0	1205 bp	1.0	80.3
rpl14_p5	TCAGCAATAACGTCTCTGCC	ATTGAGATAGCTGCGTGCAA	50.0	45.0	58.0	58.0	760 bp	0.25	49.7