Hotspot

ccsA-ndhD

SSC · 115121-115343 · 223 bp

Back to Sansevieria

Pi 0.0321

MarkerSeek score 51.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 12
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGACCCCAAAGAAACTTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.9	58.0	793 bp	1.0	80.6
ccsA-ndhD_p2	AGTGCCATTTTTACCCAGGG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.1	58.0	1246 bp	1.0	80.3
ccsA-ndhD_p3	GCCCCAAGTGCCATTTTTAC	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.9	58.0	1252 bp	1.0	80.0
ccsA-ndhD_p4	AGTGCCATTTTTACCCAGGG	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.1	58.0	1244 bp	1.0	80.0
ccsA-ndhD_p5	GTTTCACCACATCGGGTCTT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.1	58.0	1227 bp	1.0	79.9