Hotspot

rpl32-trnL-UAG

SSC · 113373-113990 · 618 bp

Back to Sansevieria

Pi 0.0166

MarkerSeek score 58.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 618
variable_sites: 16
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGTATTTAGCTGCAATAAAAGC	AGCGTGTCTACCGATTTCAC	37.5	50.0	57.9	58.0	750-1056 bp	1.0	48.7
rpl32-trnL-UAG_p2	AGGGTATTTAGCTGCAATAAAAGC	CAGCGTGTCTACCGATTTCA	37.5	50.0	57.9	58.0	751-1057 bp	1.0	48.7
rpl32-trnL-UAG_p3	AGGGTATTTAGCTGCAATAAAAGC	TTTACAGTATGCCGCCACTC	37.5	50.0	57.9	58.0	810-1116 bp	1.0	48.5
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAGA	AGCGTGTCTACCGATTTCAC	35.0	50.0	53.8	58.0	678-984 bp	1.0	48.2
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAGA	CAGCGTGTCTACCGATTTCA	35.0	50.0	53.8	58.0	679-985 bp	1.0	48.2