Hotspot

infA-rps8

LSC · 79755-79768 · 14 bp

Back to Sansevieria

Pi 0.0357

MarkerSeek score 62.10

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 14
variable_sites: 1
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GGTATCAAACTGCGTCGGAT	CTTTCCACTTCTCGAGGCAT	50.0	50.0	58.1	57.6	218 bp	1.0	76.7
infA-rps8_p2	TCTAAAAGGACTCGGACCGT	CTTTCCACTTCTCGAGGCAT	50.0	50.0	58.1	57.6	161 bp	1.0	76.7
infA-rps8_p3	GGTATCAAACTGCGTCGGAT	TTCTTTCCACTTCTCGAGGC	50.0	50.0	58.1	57.5	220 bp	1.0	76.2
infA-rps8_p4	TCTAAAAGGACTCGGACCGT	TTCTTTCCACTTCTCGAGGC	50.0	50.0	58.1	57.5	163 bp	1.0	76.2
infA-rps8_p5	GACCGTTGGGAAGTGATTCA	CTTTCCACTTCTCGAGGCAT	50.0	50.0	57.8	57.6	147 bp	1.0	76.0