Hotspot

trnP-UGG-psaJ

LSC · 66540-66899 · 360 bp

Back to Sansevieria

Pi 0.00833

MarkerSeek score 42.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 6
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AGGCGCTGTGGAAAGATATG	50.0	50.0	58.1	58.0	450-451 bp	1.0	80.1
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	CAGGCGCTGTGGAAAGATAT	50.0	50.0	58.1	58.0	451-452 bp	1.0	80.1
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	AGGCGCTGTGGAAAGATATG	50.0	50.0	58.1	58.0	449-450 bp	1.0	80.1
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	CAGGCGCTGTGGAAAGATAT	50.0	50.0	58.1	58.0	450-451 bp	1.0	80.1
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	AGGCGCTGTGGAAAGATATG	50.0	50.0	58.1	58.0	461-462 bp	1.0	80.0