Hotspot

psaA-ycf3

LSC · 41327-41968 · 642 bp

Back to Sansevieria

Pi 0.00234

MarkerSeek score 51.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 3
pi: 0.00234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	820-821 bp	1.0	79.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	818-819 bp	1.0	78.7
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	747-748 bp	1.0	78.4
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	751-752 bp	1.0	78.1
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	746-747 bp	1.0	77.4