Hotspot

trnK-UUU-rps16

LSC · 3937-4563 · 627 bp

Back to Sansevieria

Pi 0.00985
MarkerSeek score 51.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
627
variable_sites
12
pi
0.00985
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AATGTTCCCGCCATTCGTTA 50.0 45.0 58.2 57.9 762-772 bp 1.0 80.1
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TGTTCCCGCCATTCGTTATT 50.0 45.0 58.2 57.9 760-770 bp 1.0 80.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AATGTTCCCGCCATTCGTTA 50.0 45.0 58.2 57.9 761-771 bp 1.0 80.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TAAATGTTCCCGCCATTCGT 50.0 45.0 58.2 57.9 763-773 bp 1.0 80.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGTTCCCGCCATTCGTTATT 50.0 45.0 58.2 57.9 759-769 bp 1.0 80.1