Hotspot

ycf1

SSC · 126004-131316 · 5313 bp

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Pi 0.006

MarkerSeek score 54.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 172
pi: 0.006
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTGGATTTCCTTTTGAGAGTTCT	TTTTAACGGTTTGGGGAACG	37.5	45.0	57.4	56.8	229-262 bp	0.952381	46.4
ycf1_p2	TGGATTTCCTTTTGAGAGTTCT	TTTTAACGGTTTGGGGAACG	36.4	45.0	55.3	56.8	227-260 bp	0.952381	46.4
ycf1_p3	GTTGGATTTCCTTTTGAGAGTTCT	TTTAACGGTTTGGGGAACGC	37.5	50.0	57.4	59.3	228-261 bp	0.952381	46.4
ycf1_p4	TGGATTTCCTTTTGAGAGTTCT	TTTAACGGTTTGGGGAACGC	36.4	50.0	55.3	59.3	226-259 bp	0.952381	46.4
ycf1_p5	GTTGGATTTCCTTTTGAGAGTTCT	TTTTTAACGGTTTGGGGAACG	37.5	42.9	57.4	57.6	230-263 bp	0.952381	46.4