Hotspot

ndhF-rpl32

SSC · 114608-115211 · 604 bp

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Pi 0.0069
MarkerSeek score 64.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
604
variable_sites
25
pi
0.0069
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG AAGCTGCCCAATATCCCTTC 50.0 50.0 58.0 57.6 773-793 bp 0.952381 79.7
ndhF-rpl32_p2 GTTGCCGACGGAAAAAGAAG TGCCCAATATCCCTTCCCTT 50.0 50.0 58.0 58.4 769-789 bp 0.952381 79.6
ndhF-rpl32_p3 TGCCGACGGAAAAAGAAGAA AAGCTGCCCAATATCCCTTC 45.0 50.0 58.0 57.6 771-791 bp 0.952381 79.6
ndhF-rpl32_p4 TTGCCGACGGAAAAAGAAGA AAGCTGCCCAATATCCCTTC 45.0 50.0 58.0 57.6 772-792 bp 0.952381 79.6
ndhF-rpl32_p5 TGCCGACGGAAAAAGAAGAA TGCCCAATATCCCTTCCCTT 45.0 50.0 58.0 58.4 767-787 bp 0.952381 79.5