Hotspot

rpl22-rps19

LSC · 85832-85898 · 67 bp

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Pi 0.0132

MarkerSeek score 60.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 4
pi: 0.0132
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGATCAACTACTCTTCGCGC	AAATTTCCGAGGACATGCGA	50.0	45.0	58.1	57.9	204-264 bp	1.0	83.3
rpl22-rps19_p2	TGATCAACTACTCTTCGCGC	AATTTCCGAGGACATGCGAA	50.0	45.0	58.1	57.9	203-263 bp	1.0	83.0
rpl22-rps19_p3	TGATCAACTACTCTTCGCGC	ATTTCCGAGGACATGCGAAA	50.0	45.0	58.1	57.9	202-262 bp	1.0	83.0
rpl22-rps19_p4	TGATCAACTACTCTTCGCGC	TTTCCGAGGACATGCGAAAA	50.0	45.0	58.1	58.1	201-261 bp	1.0	83.0
rpl22-rps19_p5	ACTACTCTTCGCGCTTTGTT	ATTTCCGAGGACATGCGAAA	45.0	45.0	57.8	57.9	196-256 bp	1.0	82.6