Hotspot

psaJ-rpl33

LSC · 68924-69352 · 429 bp

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Pi 0.00682

MarkerSeek score 61.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 20
pi: 0.00682
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	CCATGAACCCCCTTTGGATT	45.0	50.0	58.0	57.7	487-513 bp	1.0	85.0
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	CCATGAACCCCCTTTGGATT	45.0	50.0	58.1	57.7	478-504 bp	1.0	84.6
psaJ-rpl33_p3	TTTTCCCAGATGCGTTGACA	ACACCGTTTCGAACACAACT	45.0	45.0	58.0	58.3	551-577 bp	1.0	84.5
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	ACACCGTTTCGAACACAACT	45.0	45.0	58.1	58.3	542-568 bp	1.0	84.1
psaJ-rpl33_p5	TTCCCAGATGCGTTGACATT	CCATGAACCCCCTTTGGATT	45.0	50.0	57.8	57.7	485-511 bp	1.0	83.6