Hotspot

psbE-petL

LSC · 66692-67608 · 917 bp

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Pi 0.00818

MarkerSeek score 66.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 917
variable_sites: 42
pi: 0.00818
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CACAGCAACGCACTTTCAAT	AGTCGTATTTTGCTCAGACCA	45.0	42.9	57.9	57.3	852-1036 bp	1.0	72.9
psbE-petL_p2	CAGCAACGCACTTTCAATCA	AGTCGTATTTTGCTCAGACCA	45.0	42.9	57.6	57.3	850-1034 bp	1.0	70.7
psbE-petL_p3	CACAGCAACGCACTTTCAAT	AGACGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.0	805-989 bp	1.0	70.7
psbE-petL_p4	ACAGCAACGCACTTTCAATC	AGTCGTATTTTGCTCAGACCA	45.0	42.9	57.6	57.3	851-1035 bp	1.0	70.6
psbE-petL_p5	CACAGCAACGCACTTTCAAT	AGTCGTATTTTGCTCAGACCAA	45.0	40.9	57.9	58.0	852-1036 bp	1.0	70.3