Hotspot

petA-psbJ

LSC · 64655-65730 · 1076 bp

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Pi 0.00673

MarkerSeek score 60.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 41
pi: 0.00673
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1197-1232 bp	1.0	83.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1184-1219 bp	1.0	83.4
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1198-1233 bp	1.0	83.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1185-1220 bp	1.0	82.8
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	CCGGATTGGGTTCATCTCTG	55.0	55.0	57.7	58.0	1202-1237 bp	1.0	82.1