Hotspot

trnH(GTG)-psbA

LSC · 75-480 · 406 bp

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Pi 0.0221
MarkerSeek score 74.00
Primer available yes
Label trnH(GTG)-psbA

Feature Metadata

label_name
trnH(GTG)-psbA
feature_type
igs
region
LSC
length_bp
406
variable_sites
41
pi
0.0221
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GTG)-psbA_p1 GATCCACTTGGCTACATCCG TGGAAGTTATGCACGAACGT 55.0 45.0 58.1 57.8 493-520 bp 1.0 88.4
trnH(GTG)-psbA_p2 CAATCCACTGCCTTGATCCA TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 507-534 bp 1.0 88.1
trnH(GTG)-psbA_p3 AATCCACTGCCTTGATCCAC TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 506-533 bp 1.0 88.1
trnH(GTG)-psbA_p4 ACAATCCACTGCCTTGATCC TGGAAGTTATGCACGAACGT 50.0 45.0 57.9 57.8 508-535 bp 1.0 88.1
trnH(GTG)-psbA_p5 GATCCACTTGGCTACATCCG TGCACGAACGTAATGCTCAT 55.0 45.0 58.1 58.3 484-511 bp 1.0 87.5