Hotspot

ycf1

SSC · 125580-131294 · 5715 bp

Back to Sanguisorba

Pi 0.0166

MarkerSeek score 59.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5715
variable_sites: 420
pi: 0.0166
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCGTTTTCTCGTCGGGAAG	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.0	58.0	1064-1100 bp	0.833333	80.9
ycf1_p2	ATCGTTTTCTCGTCGGGAAG	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.0	58.0	1050-1086 bp	0.833333	80.8
ycf1_p3	ATCGTTTTCTCGTCGGGAAG	AGAAGATCCTTCCCCTTCCC	50.0	55.0	58.0	58.1	1026-1062 bp	0.833333	80.6
ycf1_p4	ATCGTTTTCTCGTCGGGAAG	GAAGATCCTTCCCCTTCCCT	50.0	55.0	58.0	58.1	1025-1061 bp	0.833333	80.6
ycf1_p5	ATCGTTTTCTCGTCGGGAAG	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	1174-1210 bp	0.833333	80.2