Hotspot

psaJ-rpl33

LSC · 68299-68768 · 470 bp

Back to Sanguisorba

Pi 0.0192

MarkerSeek score 71.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 40
pi: 0.0192
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	413-557 bp	1.0	87.4
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	TCTGGAAATGCCCGTTGATT	50.0	45.0	57.8	57.8	487-612 bp	0.916667	80.7
psaJ-rpl33_p3	GTTCGGGTCTTTAGCAGGTT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	458-583 bp	0.916667	80.7
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	TCTGGAAATGCCCGTTGATT	50.0	45.0	57.8	57.8	532-657 bp	0.916667	80.5
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	TCTGGAAATGCCCGTTGATT	50.0	45.0	57.8	57.8	557-682 bp	0.916667	80.1