Hotspot

ndhC-trnV-UAC

LSC · 52234-52901 · 668 bp

Back to Sanguisorba

Pi 0.0368
MarkerSeek score 73.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
668
variable_sites
90
pi
0.0368
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTTTCCGGCCCTTTGCTAAT AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.1 57.8 683-826 bp 1.0 89.9
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 662-805 bp 1.0 89.3
ndhC-trnV-UAC_p3 TTTTTCCGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.3 57.8 684-827 bp 1.0 88.1
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 674-817 bp 1.0 87.7
ndhC-trnV-UAC_p5 TTTTCCGGCCCTTTGCTAAT GAAGGTCTACGGTTCGAGTC 45.0 55.0 58.1 57.5 682-825 bp 1.0 87.1