Hotspot

ycf3-trnS-GGA

LSC · 45160-46045 · 886 bp

Back to Sanguisorba

Pi 0.0149

MarkerSeek score 69.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 60
pi: 0.0149
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	639-1106 bp	1.0	86.1
ycf3-trnS-GGA_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	638-1105 bp	1.0	86.1
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTCAA	50.0	45.0	57.8	57.8	589-1056 bp	1.0	86.0
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTCAA	50.0	45.0	57.8	57.8	588-1055 bp	1.0	86.0
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTC	50.0	50.0	57.8	58.3	591-1058 bp	1.0	85.5