Hotspot

petN-psbM

LSC · 28438-29665 · 1228 bp

Back to Sanguisorba

Pi 0.0164

MarkerSeek score 71.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 94
pi: 0.0164
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1310-1356 bp	0.916667	77.8
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	AAACAGTCAGCCAAGGTGAT	50.0	45.0	58.0	57.3	1309-1355 bp	0.916667	76.0
petN-psbM_p3	AGTAAATCTCGCTTGGGCTG	AACAGTCAGCCAAGGTGATT	50.0	45.0	58.0	57.3	1308-1354 bp	0.916667	76.0
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AAAACAGTCAGCCAAGGTGA	50.0	45.0	57.6	57.6	1250-1296 bp	0.916667	75.5
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AACAGTCAGCCAAGGTGATT	50.0	45.0	57.6	57.3	1248-1294 bp	0.916667	73.7