Hotspot

rpoB-trnC-GCA

LSC · 26418-27489 · 1072 bp

Back to Sanguisorba

Pi 0.0171

MarkerSeek score 71.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 74
pi: 0.0171
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TATTGCCTCATTTCGACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1127-1161 bp	1.0	84.7
rpoB-trnC-GCA_p2	TATTGCCTCATTTCGACCCC	GACACCCGGATTTGAACTGG	50.0	55.0	57.7	58.8	1132-1166 bp	1.0	78.6
rpoB-trnC-GCA_p3	CATTTCGACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.1	58.1	1119-1153 bp	1.0	78.6
rpoB-trnC-GCA_p4	ATATTGCCTCATTTCGACCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.1	58.1	1128-1162 bp	1.0	78.3
rpoB-trnC-GCA_p5	TATTGCCTCATTTCGACCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1109-1143 bp	0.916667	76.7