Hotspot

atpF-atpH

LSC · 12721-13200 · 480 bp

Back to Sanguisorba

Pi 0.0138

MarkerSeek score 68.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 35
pi: 0.0138
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAAGGAAAAATACGGGGT	50.0	50.0	57.9	57.9	643-667 bp	1.0	86.6
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CCAGAGGCGGAAGGAAAAAT	50.0	50.0	57.9	58.2	650-674 bp	1.0	86.2
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	AGGCGGAAGGAAAAATACGG	50.0	50.0	57.9	58.3	646-670 bp	1.0	85.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	579-603 bp	1.0	84.7
atpF-atpH_p5	GATGGCCAATAACCCAAGGA	CGGAAGGAAAAATACGGGGT	50.0	50.0	57.5	57.9	621-645 bp	1.0	83.9