Hotspot

trnR-UCU-atpA

LSC · 10087-10591 · 505 bp

Back to Sanguisorba

Pi 0.0348

MarkerSeek score 70.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 67
pi: 0.0348
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATTGGAGTGAAAAGCGTCCA	GACCAGATGGACCGTTTTCT	45.0	50.0	57.7	57.5	599-629 bp	1.0	86.0
trnR-UCU-atpA_p2	TGGAGTGAAAAGCGTCCATT	GACCAGATGGACCGTTTTCT	45.0	50.0	57.7	57.5	597-627 bp	1.0	86.0
trnR-UCU-atpA_p3	TTGGAGTGAAAAGCGTCCAT	GACCAGATGGACCGTTTTCT	45.0	50.0	57.7	57.5	598-628 bp	1.0	86.0
trnR-UCU-atpA_p4	TGGAGTGAAAAGCGTCCATT	GGACCAGATGGACCGTTTTC	45.0	55.0	57.7	58.5	598-628 bp	1.0	85.4
trnR-UCU-atpA_p5	TTGGAGTGAAAAGCGTCCAT	GGACCAGATGGACCGTTTTC	45.0	55.0	57.7	58.5	599-629 bp	1.0	85.4