Hotspot

trnH-GUG-psbA

LSC · 101-416 · 316 bp

Back to Sanguisorba

Pi 0.0645
MarkerSeek score 76.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
316
variable_sites
73
pi
0.0645
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 353-421 bp 1.0 90.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 367-435 bp 1.0 90.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 366-434 bp 1.0 90.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 368-436 bp 1.0 90.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 356-424 bp 1.0 90.4