Hotspot

rpl32-trnL-UAG

SSC · 117286-118218 · 933 bp

Back to Sambucus

Pi 0.0108
MarkerSeek score 64.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
933
variable_sites
30
pi
0.0108
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 905-1079 bp 1.0 85.3
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 906-1080 bp 1.0 85.3
rpl32-trnL-UAG_p3 AAAAGGAAGGGGTATTGGGC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 922-1096 bp 1.0 84.3
rpl32-trnL-UAG_p4 AAAAGGAAGGGGTATTGGGC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 923-1097 bp 1.0 84.3
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 915-1089 bp 1.0 84.3