Hotspot

ndhC-trnV-UAC

LSC · 52923-54113 · 1191 bp

Back to Sambucus

Pi 0.00796

MarkerSeek score 66.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1191
variable_sites: 25
pi: 0.00796
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGCTAATCGGGGCTAAAACT	CCGAGAATGTCTACGGTTCG	50.0	55.0	58.1	58.2	1209-1353 bp	1.0	83.7
ndhC-trnV-UAC_p2	TCGCTAATCGGGGCTAAAAC	CCGAGAATGTCTACGGTTCG	50.0	55.0	58.1	58.2	1210-1354 bp	1.0	83.7
ndhC-trnV-UAC_p3	CTCTGGACCTTCGCTAATCG	CCGAGAATGTCTACGGTTCG	55.0	55.0	57.9	58.2	1220-1364 bp	1.0	83.1
ndhC-trnV-UAC_p4	GGGCTAAAACTCCGGAAAGT	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.8	58.2	1200-1344 bp	1.0	82.6
ndhC-trnV-UAC_p5	CTTCGCTAATCGGGGCTAAA	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.8	58.2	1212-1356 bp	1.0	82.2