Hotspot

trnF-GAA-ndhJ

LSC · 50548-51242 · 695 bp

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Pi 0.0149

MarkerSeek score 58.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 39
pi: 0.0149
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	820-839 bp	1.0	75.3
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	819-838 bp	1.0	75.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	784-803 bp	1.0	75.2
trnF-GAA-ndhJ_p4	ATGTGGCATCGGTAATGGTC	TGGATAGGATGGCCTTTACG	50.0	50.0	58.0	56.8	840-859 bp	1.0	75.0
trnF-GAA-ndhJ_p5	ATGTGGCATCGGTAATGGTC	GGATAGGATGGCCTTTACGT	50.0	50.0	58.0	56.8	839-858 bp	1.0	75.0