Hotspot

trnT-UGU-trnL-UAA

LSC · 48719-49506 · 788 bp

Back to Sambucus

Pi 0.00995
MarkerSeek score 64.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 811-856 bp 1.0 85.2
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 819-864 bp 1.0 84.7
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 843-888 bp 1.0 84.3
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 851-896 bp 1.0 83.7
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 825-870 bp 1.0 83.3