Hotspot

psbC-trnS-UGA

LSC · 37188-37418 · 231 bp

Back to Sambucus

Pi 0.0199

MarkerSeek score 63.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 14
pi: 0.0199
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	389-404 bp	1.0	82.9
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	350-365 bp	1.0	82.8
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.1	58.1	381-396 bp	1.0	82.7
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.1	58.1	382-397 bp	1.0	82.7
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	374-389 bp	1.0	82.7