Hotspot

trnE-UUC-trnT-GGU

LSC · 32425-33262 · 838 bp

Back to Sambucus

Pi 0.0219

MarkerSeek score 73.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 73
pi: 0.0219
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AACTTCCTTTCGCAGTACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	953-994 bp	1.0	88.5
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	887-928 bp	1.0	88.1
trnE-UUC-trnT-GGU_p3	AACTTCCTTTCGCAGTACCC	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	950-991 bp	1.0	87.4
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	884-925 bp	1.0	87.0
trnE-UUC-trnT-GGU_p5	AACTTCCTTTCGCAGTACCC	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	955-996 bp	1.0	86.3