Hotspot

rps2-rpoC2

LSC · 17301-17533 · 233 bp

Back to Sambucus

Pi 0.0237

MarkerSeek score 61.00

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 13
pi: 0.0237
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ATCGTTCGCTGGAATCGAAA	GGTTGAGGGTTGGAACGAAT	45.0	50.0	58.0	58.1	1518-1523 bp	1.0	80.7
rps2-rpoC2_p2	TCGTTCGCTGGAATCGAAAT	GGTTGAGGGTTGGAACGAAT	45.0	50.0	58.0	58.1	1517-1522 bp	1.0	80.7
rps2-rpoC2_p3	ATCGTTCGCTGGAATCGAAA	CTCGAAAAGAGGGTTGAGGG	45.0	55.0	58.0	57.9	1529-1534 bp	1.0	80.6
rps2-rpoC2_p4	TCGTTCGCTGGAATCGAAAT	CTCGAAAAGAGGGTTGAGGG	45.0	55.0	58.0	57.9	1528-1533 bp	1.0	80.6
rps2-rpoC2_p5	CGAGATCGGGGTCACAATTT	GGTTGAGGGTTGGAACGAAT	50.0	50.0	58.0	58.1	1492-1497 bp	1.0	80.6